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Fig. 3.
Formation and characterization of follicle-like structures. (A) Media
estradiol levels (pg/ml) from cultures between d7 and d34. (B and C)
Representative phase contrast images of structures that are morphologically
similar to early primary or secondary follicles. (D) Quantitative
RT-PCR analysis of GDF-9 in d16 and d22 cultures and in d16F-replated
cultures. (E), (F), and (H) depict aggregate cultures
and show that many of the follicle-like structures maintain their
three-dimensional organization. (F) is a magnified representation of (E) at a
different area of the same culture. (I) represents an adherent culture; all
other panels show nonadherent cells and structures. (G) Quantitative
RT-PCR analysis of aromatase, CYP17, and StAR in d16 and d22
cultures and in d16F-replated cultures. (I) Phase contrast image of
oocyte-like cells released from d26 culture. All pictures are whole mount
stainings. Bar scales: 15 µm in (B), 30 µm in (C), 500 µm in (E), 50 µm in
(F), 40 µm in (H), and 100 µm in (I).
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