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Figure 1. Scheme
for Therapeutic Cloning Combined with Gene and Cell Therapy. A piece of tail
from a mouse homozygous for the recombination-activating gene 2 (Rag2)
mutation was removed and cultured. After fibroblast-like cells grew out, they
were used as donors for nuclear transfer by direct injection into enucleated
MII oocytes using a Piezoelectric-driven micromanipulator. Embryonic stem
(ES) cells isolated from the NT-derived blastocysts were genetically repaired
by homologous recombination. After repair, the ntES cells were differentiated
in vitro into embryoid bodies (EBs), infected with the HoxB4iGFP retrovirus,
expanded, and injected into the tail vein of irradiated, Rag2-deficient mice.
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