特別演講1:

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P-3
Personal Regulome Navigation
Howard Y. Chang, M.D., Ph.D.
Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, CA 94305.

  In every human cell, two meters of DNA is packed into a ~10 micron nucleus. The genome is extensively compacted, except for the active regulatory DNA elements that remain accessible. We have developed an Assay of Transpose Accessible Chromatin by sequencing (ATAC-seq) as a facile and sensitive method to map the landscape of gene regulation. The landscape of accessible chromatin from primary cells and tissues is unique informative for cell types, cell states, and the molecular basis of genetic variants associated with human diseases. Single cell chromatin accessibility reveals DNA elements and trans-acting factors that mediate cell-to-cell variation, which directly impacts cancer heterogeneity. Real time monitoring of gene regulation landscape in patients can provide prognostic prediction for response to cancer therapy.? New technologies that incorporate 3-dimensional chromosome conformation, in situ imaging, and single cell multi-omics provide new levels of precision and insights into human diseases.